理化学研究所筑波ライフサイエンスセンター・八木　健先生（1993）。TT2 ES細胞を用いて作出。C57BL/6へ戻し交配された。 Takeshi YAGI Developed by Takeshi Yagi, Tsukuba Life Science Center, RIKEN in 1993. The targeting vector containing lacZ-neo cassette was transferred into TT2 ES cells to replace the SH1, SH3 and tyrosine kinase regions of the Fyn gene. The mutant mice were crossed to C57BL/6. B6.Cg-Fyn<tm1Yik>, Fyn Targeting mice B6.Cg-Fyn<tm1Yik>, Fyn Targeting mice 非受容体型チロシンキナーゼFyn遺伝子のノックアウトマウス。ホモマウスは海馬の構造異常、恐怖反応の亢進、痙攣感受性の亢進、エタノール感受性の亢進が見られる。交配・繁殖は正常。 C (3-6 months) TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 366, 742-745 (1993).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.<br>When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration. <a href='https://brc.riken.jp/mus/pcr01000'>Genotyping protocol -PCR-</a> RBRC01000 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 366, 742-745 (1993).<br>本件リソースの特許権ならびに知的所有権は寄託者が保持しており、利用者が本件リソースから得られた結果により特許等を申請する場合には、事前に寄託者と協議する。利用者は本件リソースを利用して寄託後2年間以内に行った研究結果等を学術論文発表する場合には寄託者との共著の要否について別途協議する。 岡崎国立共同研究機構 E. coli lacZ, polyA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Fyn genomic DNA Natl. Inst. Basic Biol.,Okazaki Inst. Integrative Bioscience B6.Cg-Fyn<tm1Yik>/TyagRbrc B6.Cg-Fyn<tm1Yik>/TyagRbrc 非受容体型チロシンキナーゼFyn遺伝子のノックアウトマウス。ホモマウスは海馬の構造異常、恐怖反応の亢進、痙攣感受性の亢進、エタノール感受性の亢進が見られる。交配・繁殖は正常。, C（3〜6か月） Fyn gene knockout mice. Fyn proto-oncogene is a non-receptor type tyrosine kinase of Src family that is expressed in the central nervous system and suggested to be involved in the mechanism of neuronal signaling cascade. Homozygous mutant mice exhibit a defect in suckling behavior and abnormal hippocampus morphology. This strain is useful for elucidation of the role of Fyn tyrosine kinase. 八木　健 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.fbs.osaka-u.ac.jp/labs/yagi/index.htm" target="_blank">Lab HP (Japanese)</A> true Fluorescent Proteins/lacZ System